Composite
Part:BBa_K4273016:Design
Designed by: Su Junzhe Group: iGEM22_LINKS_China (2022-09-30)
pTDH3-xyl1-tTDH1-pPGK1-xyl2-tPGK1-pTEF2-xyl3-tSSA1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 770
Illegal BglII site found at 1159
Illegal BglII site found at 2994
Illegal BglII site found at 5119
Illegal XhoI site found at 1646
Illegal XhoI site found at 6620 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 790
Illegal AgeI site found at 3123
Illegal AgeI site found at 3699
Illegal AgeI site found at 5167 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1892
Illegal BsaI site found at 6606
Illegal BsaI.rc site found at 3004
Illegal BsaI.rc site found at 6856
Illegal SapI site found at 4764
Illegal SapI.rc site found at 5434
Design Notes
Considered to be functional in Saccharomyces cerevisiae
Source
Scheffersomyces stipitis