Composite

Part:BBa_K4273016:Design

Designed by: Su Junzhe   Group: iGEM22_LINKS_China   (2022-09-30)


pTDH3-xyl1-tTDH1-pPGK1-xyl2-tPGK1-pTEF2-xyl3-tSSA1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 770
    Illegal BglII site found at 1159
    Illegal BglII site found at 2994
    Illegal BglII site found at 5119
    Illegal XhoI site found at 1646
    Illegal XhoI site found at 6620
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 790
    Illegal AgeI site found at 3123
    Illegal AgeI site found at 3699
    Illegal AgeI site found at 5167
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1892
    Illegal BsaI site found at 6606
    Illegal BsaI.rc site found at 3004
    Illegal BsaI.rc site found at 6856
    Illegal SapI site found at 4764
    Illegal SapI.rc site found at 5434


Design Notes

Considered to be functional in Saccharomyces cerevisiae


Source

Scheffersomyces stipitis

References